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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 2;189(12):5533–5540. doi: 10.4049/jimmunol.1201161

Figure 6. Conditioned media from MIF expressing 4T1 cells induces differentiation of monocytic MDSCs.

Figure 6

CD11b+ cells were isolated from the spleen of WT tumor-bearing mice and incubated with conditioned media from MIF non-targeting (NT) control cells or MIF KD 4T1 cells with or without SFN as indicated for 5 days (schematic of experiment in Supplemental Figure 3A). A, Phenotype of purified CD11b cells at start of experiment, stained as in Figure 4A n=3. B, Phenotype of cells after 5 days of culture. Cells were stained for analysis by flow cytometry as in Figure 4A. n>12 for each group and includes results of 4 independent experiments. * p<0.05 by ANOVA.