4T1 non-targeting control (NT) or MIF depleted (KD) cells were implanted as in Figure 2. Mice were injected intraperitoneally with either rat IgG as a control or GR1 antibody on days 11, 14, and 17. A, MDSC depletion in WT tumor-bearing mice decreases tumor growth, while MDSC depletion in KD tumor-bearing mice does not. n>6 for each group and includes 2 independent experiments. B, MDSC depletion in WT tumor-bearing mice decreases metastasis. Lungs were excised at day 20, and metastasis was quantified using the clonogenic assay. n>6 for each group and includes 2 independent experiments. *= p<0.05 by ANOVA.