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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 5;189(12):5886–5895. doi: 10.4049/jimmunol.1103092

Figure 1. ManLAM pretreatment desensitizes naïve human T cell migration to S1P.

Figure 1

T cells from 3 healthy donors were treated with (A) 1mg/mL whole irradiated H37Rv, (B) 100ng/mL ManLAM or (C) 100ng/mL PIM6 for 2 hours before use in Boyden chamber assays for media control, S1P (10nM), or CCL21 (50ng/ml). Migrating cells were fixed and stained, and number of T cells counted at 400x magnification from at least 10 fields were normalized to media control. irH37Rv inhibited S1P directed migration but not CCL21 directed migration (*P=0.02, Student’s t test). (B) Pretreatment with 100ng/mL ManLAM also blocks S1P directed migration (**P=0.007 Student’s t test). Data in (A & B) were obtained from 3 donors assayed in duplicate (mean ± s.e.m.). (C) 100ng/mL PIM6 had no effect on human T lymphocyte migration induced by S1P (n=2 donors assayed in duplicate, mean ± s.e.m.).