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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 7;189(12):5659–5666. doi: 10.4049/jimmunol.1202313

Figure 4.

Figure 4

Cardiac allograft response in wild-type vs. CDK2-deficient mice. Wild-type (squares) or CDK2-deficient (circles) mice were transplanted with BALB/c cardiac grafts, treated with control Ig (n=3, black lines) or CTLA-4Ig (n=6–7, colored lines), and graft survival was observed over a 100 day period (A). In a separate experiment, groups of three wild-type or CDK2-deficient mice were transplanted and treated as in A, and sacrificed at day 32 post-transplant. p values were calculated by ANOVA. CD4+ T cells purified from the spleens of control Ig-treated (B) and CTLA-4Ig-treated (C) recipients were stimulated with BALB/c splenocytes in vitro, and the frequency of donor-specific cells was determined by ELISPOT. Allograft tissue harvested from CTLA-4Ig-treated recipients was sectioned and stained with H&E (D), antibodies against CD4, CD8, and Foxp3 (E), or antibodies against CD3, CDK2 and cyclin A (F).