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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Oct 17;1833(1):1–10. doi: 10.1016/j.bbamcr.2012.10.011

Figure 3. Nuclear localization, but not transamidase activity, of TG2 is required to suppress HIF activity in different human cell lines.

Figure 3

(a) Nuclear localization of TG2 is required to suppress HIF activity in HEK cells. Relative HRE luciferase activity in HEK-293A cells transiently transfected with TG2 constructs after 15 h of 0.1% oxygen treatment. Data is presented as percent of empty vector controls. NLS-tagged TG2 significantly suppressed HRE activity; whereas untagged and NES-tagged TG2 had no effect. The effect of the transamidating inactive TG2 (W241A-TG2) was the same as wild type TG2 (N=4). (b) Nuclear localization of TG2 is required to suppress HIF activity in MCF-7 cells. Relative HRE luciferase activity in MCF-7 cells transiently transfected with TG2 constructs after 15 h of 0.1% oxygen treatment. Data is presented as percent of empty vectors. NES-tagged TG2 significantly induced HRE activity. The effect of the transamidating inactive TG2 (W241A-TG2) was the same as wild type TG2 (N=4). Results are shown as mean ± SEM *p<0.05, **p<0.01.