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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Oct 17;1833(1):1–10. doi: 10.1016/j.bbamcr.2012.10.011

Figure 4. All domains of TG2 are required for maximal transamidase activity.

Figure 4

(a) The schematic representation of TG2 deletions used. The TG2 deletions were based on the well-defined domains of TG2 which were determined by its crystal structure [48]. (b) N-terminal deletion causes complete and C-terminal deletion causes partial loss of transamidase activity of TG2. HEK-293A cells were transfected with V5-tagged TG2 deletion constructs and the cells were lysed after 48 h. Transamidase activity in the lysates was detected by a ELISA based assay and the results are presented as percent of full length TG2. (N=4). Results are shown as mean ± SEM.