Primary epithelial cells from FT, EM, Cx, and Ecx were treated with poly(I:C) for 24 hr. Apical and basolateral conditioned media were collected and assayed for IL-8 secretion by ELISA. Data from one representative patient sample from each compartment is shown. Apical secretions are shown in black and basolateral secretions are shown in white. The p values were calculated by comparing triplicate wells for Control and Treatment, using a two-tailed paired t test. Significance denoted by *; *, p<0.05; **, p<0.01; ***, p<0.001.