Fig. 3.
Representative photomicrographs of 5mC staining (blackish color in cell nuclei) in fetal membranes (FM; fetal placenta; chorioallantois) and endometrium (E; maternal placenta) in (A) NAT, (B) NAT-ET, (C) IVF and (D) IVA groups on Day 22 of pregnancy. The reddish/pinkish staining represents nuclear fast red counterstaining. Note a very low 5mC expression in some cells (arrows) in each group (A–D). In the inset in (B), note a lack of positive staining in the control sections in which mouse IgG was used in place of the primary antibody.