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. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: J Neuroimmune Pharmacol. 2012 Oct 19;7(4):991–1001. doi: 10.1007/s11481-012-9403-y

Mice were exposed to PCB153-NPs by infusion into the internal carotid artery (ICA) at the dose of 5 ng PCB153/g body weight bound to 1.04 × 10⁵ silica NPs. Control mice were infused with the same amounts of NPs, PCB153 dissolved in DMSO, or vehicle (PBS or 0.01% DMSO). Brain microvessels were isolated 24 h post treatment and analyzed for occludin (A) and claudin-5 (B) expression by immunoblotting and immunofluorescence. Immunoreactivity of occludin and claudin-5 was stained in green and red, respectively; scale bar=20 μm. The blots in A and B are representative images from all experiments and the quantified results are depicted in the form of bar graphs. Arrows indicate disrupted continuity of tight junction proteins. Results are mean ± SEM, n=4. *Significantly different as compared to control groups at *p<0.05 or ***p<0.001. ^†Results in the PCB153-NP group are statistically different from those in the PCB153 group at ^†p<0.05 or ^†††p<0.001.

Mice were exposed to PCB153-NPs by infusion into the internal carotid artery (ICA) at the dose of 5 ng PCB153/g body weight bound to 1.04 × 10⁵ silica NPs. Control mice were infused with the same amounts of NPs, PCB153 dissolved in DMSO, or vehicle (PBS or 0.01% DMSO). Brain microvessels were isolated 24 h post treatment and analyzed for occludin (A) and claudin-5 (B) expression by immunoblotting and immunofluorescence. Immunoreactivity of occludin and claudin-5 was stained in green and red, respectively; scale bar=20 μm. The blots in A and B are representative images from all experiments and the quantified results are depicted in the form of bar graphs. Arrows indicate disrupted continuity of tight junction proteins. Results are mean ± SEM, n=4. *Significantly different as compared to control groups at *p<0.05 or ***p<0.001. ^†Results in the PCB153-NP group are statistically different from those in the PCB153 group at ^†p<0.05 or ^†††p<0.001.