Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2012 Oct 19;7(4):991–1001. doi: 10.1007/s11481-012-9403-y

Figure 4. Treatment with PCB153-NPs enhances monocyte transmigration.

Figure 4

Figure 4

(A) Mice were exposed to vehicle, PCB153, and/or NPs as in Figure 1, followed by injection with monocytic J774.1 cells labeled with CFDA-SE (green) into the internal carotid artery. In addition, brain sections were stained for claudin-5 (red) to visualize the vessels. Closed arrowheads indicate labeled J774.1 cells inside cerebral vessels, while open arrowheads indicate labeled J774.1 cells that appear to be present in the perivascular space. Scale bar = 20 μm. (B) Con uent hCMEC/D3 cells cultured on Transwell inserts were treated with PCB153-NPs (PCB153, 1.6 μM; NPs, 2.08 × 105), PCB153 (1.6 μM), NPs (2.08 × 105), or vehicle for 24 h. THP-1 monocytic cells were labeled with calcein AM, added on the top of endothelial monolayers, and their transendothelial migration was assessed 4 h later. Data are mean ± standard deviation (SD), n=6. *Significantly different as compared to control groups at p<0.05.