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. Author manuscript; available in PMC: 2013 Dec 10.
Published in final edited form as: ACS Comb Sci. 2012 Nov 5;14(12):680–687. doi: 10.1021/co300111f

Figure 6.

Figure 6

Activities of deoxyribozymes from the selections for DNA-catalyzed nucleopeptide linkage formation. (A) PAGE image showing representative timepoints (t = 30 s, 30 min, 2 h, and 20 h). Incubation conditions: 50 mM HEPES, pH 7.5, 20 mM MnCl2, 40 mM MgCl2, 150 mM NaCl, and 2 mM KCl at 37 °C. Substrate (open arrowhead) and product (filled arrowhead) are marked. For N30, 8TM3 was the only new deoxyribozyme identified, along with two closely related sequence variants. For N40, 11MN5 was the best deoxyribozyme identified previously by direct selection;31 data shown here were newly acquired for comparison. For N60, 7TQ20 was one of two different deoxyribozymes found, along with six close sequence variants; the other deoxyribozyme, 7TQ46 (no variants found), had slightly lower activity. (B) Kinetic plots. kobs values are tabulated in Table S4.