Fig. 6.
Effect of the CYP2E1 inhibitor diallyl sulfide (DAS, 100 mg/kg/d, i.p., for 4 weeks) on ethanol intake-induced O2− production and protein carbonyl formation. Mice were fed control or ethanol (4%) diet for 6 weeks prior to assessment of DHE fluorescence. Cohorts of ethanol consuming or non-consuming mice were administered DAS stating from week 3. A–D: Representative fluorescence images (40x) from control, ethanol, DAS and ethanol-DAS groups; E: Pooled data; and F: Protein carbonyl level. Mean ± SEM, n = 9 fields (panel E) or 7 mice (panel F) per group, * p < 0.05 vs. control group, # p < 0.05 vs. ethanol group.