Effect of the ASK-1 inhibitor thioredoxin-1 (TRX-1, 50 μM) and the JNK specific peptide inhibitor JNKI (2 μM) on ethanol exposure (240 mg/dl, 4 hrs)-induced cardiomyocyte contractile dysfunction. A: Resting cell length; B: Peak shortening (PS, normalized to cell length); C: Maximal velocity of shortening (+ dL/dt); D: Maximal of relengthening (− dL/dt); E: Time-to-PS (TPS) and F: Time-to-90% relengthening (TR90). Mean ± SEM, n = 50 cells from 2 mice per group, * p < 0.05 vs. control group, # p < 0.05 vs. ethanol group.