Table 1. Plasmids used in this study.

Plasmid	Description	Reference
pET21a	E. coli T7 expression vector	Novagen
pMAD	Allelic exchange vector	(Arnaud et al., 2004)
pPD1	pET21a derivative; 80α gp47	This work
pPD2	pET21a derivative; 80α gp46, gp47	(Poliakov et al., 2008)
pPD8	pMAD derivative; 80α Δorf46 and flanking region	This work
pJRC102	pET21a derivative; 80α gp46*, gp47*	This work
pJRC110	pET21a derivative; 80α gp47, gp46, gp42, gp44	This work
pJRC130	pET21a derivative, 80α gp47 + SaPI1 gp6, gp7	This work
pJRC131	pET21a derivative; 80α gp46, gp47 + SaPI1 gp6, gp7	This work
pG164	S. aureus T7 expression plasmid	(D'Elia et al., 2006)
pPD18	pG164 derivative; 80α gp47, native RBS§	This work
pPD20	pG164 derivative; 80α gp47	This work
pPD21	pG164 derivative; 80α gp46, gp47	This work
pEW4	pG164 derivative; 80α 47* + 46*	This work
pPD22	pG164 derivative; 80α gp42 − gp47	This work
pPD44	pG164 derivative; 80α gp47 + SaPI1 CpmB	This work
pPD45	pG164 derivative; 80α gp47 + SaPI1 CpmA	This work
pPD46	pG164 derivative; 80α gp47 + SaPI1 CpmA, CpmB	This work
pPD51	pG164 derivative; 80α gp46, gp47 + SaPI1 CpmA, CpmB	This work

^§Unless otherwise noted, all pG164-derived plasmids use the vector RBS for the first gene and native RBS for subsequent genes.