Table 3. Oligonucleotides used in this study.
Plasmid | Oligonucleotide | Sequence (5′-3′)* |
---|---|---|
pPD1 | SMT34 | GACTCATATGGAACAAACACAAAAATTAAAAT |
SMT35 | GACTGGATCCTTATTAAACTTCTCCTGGAACT | |
| ||
pPD8 | PKD-9 | GAGGATCCGGTCGAAAACAAGGACTTTAGCGATAGAG |
PKD-10 | ATGCCTCCGTTAATTTTTAATAATTCTATTTTCTTCCATGAGATATACCTCCATTTATAGTCTGTC | |
PKD-11 | ATGGAAGAAAATAGAATTATTAAAAATTAACGGAGGCATTTAAATGGAACAAAC | |
PKD-12 | GAGGATCCCAATGATTTCGGGCATGTTACCACTCC | |
| ||
pPD18 | pKD57 | AACTGCAGAACGGAGGCATTTAAATGGAAC |
pKD58 | AACTCGAGAAGTCAGGCGCGCCAATTGTTTATTAAACTTCTCCTGGAACT | |
| ||
pPD20 | PKD-78 | CACAGGATCCATGGAACAAACACAAAAATTAAAATTAAATTTGC |
PKD-58 | AACTCGAGAAGTCAGGCGCGCCAATTGTTTATTAAACTTCTCCTGGAACT | |
| ||
pPD21 | PKD-79 | CACAGGATCCATGGAAGAAAATAAACTTAAGTTTAATTTGCAA |
PKD-58 | AACTCGAGAAGTCAGGCGCGCCAATTGTTTATTAAACTTCTCCTGGAACT | |
| ||
pPD22 | PKD-66 | TTTTGGATCCATGTTAAAAGTAAACGAATTTGAAACAGATACA |
PKD-67 | AAAACTGCAGTAATTGTTTATTAAACTTCTCCTGGAACTG | |
| ||
pPD41 | PKD-104 | CACAGGATCCATGGCAGACCAATCAGATGATCCG |
PKD-58 | AACTCGAGAAGTCAGGCGCGCCAATTGTTTATTAAACTTCTCCTGGAACT | |
| ||
pPD44 | PKD-86 | GGCAAAGGCGCGCCAAGAAAGGGTAATTAAATGGAAACAAAATACGA |
PKD-87 | GGCAACTCGAGTTGCTATTTAATAATCCTGTTTTGCTTAGCTAAATTT | |
| ||
pPD45 | PKD-88 | GGCAAAGGCGCGCCAAGGGGATAAAAATGAAAACTGAATCGTAC |
PKD-89 | GGCAACTCGAGTTAACGTTTTAAAAACAACTTGTTATTGTGTTCG | |
| ||
pPD46, pPD51 | PKD-88 | GGCAAAGGCGCGCCAAGGGGATAAAAATGAAAACTGAATCGTAC |
PKD-87 | GGCAACTCGAGTTGCTATTTAATAATCCTGTTTTGCTTAGCTAAATTT |
Restriction sites are underlined