Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Liver Int. 2012 Nov 1;33(1):149–161. doi: 10.1111/liv.12016

Figure 3. Hedgehog ligands stimulate alternative macrophage activation.

Figure 3

A) mRNA expression of the alternative activation marker of macrophages Chitinase 3 like 3 (Chi3l3) in primary rat Kupffer cells treated with 10μg/ml of SEA for 6 hours. Results were normalized by mRNA expression in PBS-treated cells. B-D) mRNA expression of the alternative activation makers of macrophages (B) Chi3l3, (C) Arginase 1 (Arg1) and Fizz1 (also known as Rentlα, resistin like alpha), and (D) Hedgehog pathway target genes, Gli1 and Patched (Ptch) in RAW264.7 cells treated with DMSO ± 10 μg/ml SEA or 10μg/ml SEA with either 1μM Cyclopamine or 1μM GDC-0449 (specific antagonists of Smoothened, co-receptor of the Hedgehog pathway) for 6 hours. Results were normalized by mRNA expression in DMSO-treated cells. Means ± S.E.M. are displayed. *p<0.05; **p<0.01; †p<0.005.