Table 1.
Immunocytochemistry: primary antibodies.
| Antigen | Primary antibody | Source | Dilution |
|---|---|---|---|
| GFAP | Polyclonal rabbit | DAKO, Glostrup, Denmark | 1:4000 |
| GFAPδ. | Polyclonal rabbit | a | 1:500 |
| Vimentin | Mouse clone V9 | DAKO | 1:1000 |
| Nestin | Mouse clone 10C2 | Chemicon, Temecula, CA | 1:200 |
| Synaptophysin | Mouse clone SY38 | DAKO | 1:200 |
| Neurofilament | Mouse clone 2F11 | DAKO | 1:50 |
| HLA‐DP, DQ, DR (HLA‐II) | Mouse clone CR3/43 | DAKO | 1:100 |
| HLA class I (HLA‐I) | Mouse clone HC‐10 | b | 1:200 |
| CD68 | Mouse clone PG‐M1 | DAKO | 1:200 |
| CD3 | Mouse clone, F7.2.38 | DAKO | 1:200 |
| CD8 | Mouse clone, C8/144B | DAKO | 1:100 |
| CD4 | Mouse clone, 4B12 | Neomarkers, Duiven, The Netherlands | 1:100 |
| CD20 | Mouse clone, L26 | DAKO | 1:400 |
| Granzym B | Mouse clone, GrB‐7 | Monosan | 1:100 |
| TLR2 | Polyclonal goat | R & D systems, Abingdon UK | 1:1:00 |
| TLR4 | Polyclonal rabbit | Santa Cruz, Santa Cruz, CA, USA | 1:50 |
| Advanced glycation end products (RAGE) | Polyclonal goat | Biologo, Kronshagen, Germany | 1:200 |
| Phospho‐S6 ribosomal protein (Ser235/236; pS6) | Monoclonal rabbit | Cell Signaling Technology, Beverly, MA, USA | 1:50 |
| Phospho‐p70 S6 kinase 1 (Thr389; pS6K) | Monoclonal mouse | Cell Signaling | 1:50 |
| Phosphorylated eukaryotic initiation factor 4E binding protein 1 (Thr37/46; p4EBP1) | Monoclonal rabbit | Cell Signaling | 1:50 |
| c‐Myc | Mouse clone, 9E10 | Abcam, Cambridge, MA, USA | 1:250 |
a
Kindly provided by Dr. E.M. Hol (Department of Astrocyte Biology & Neurodegeneration, Netherlands Institute for Neuroscience).
b
Kindly provided by Dr. J. Neefjes (Netherlands Cancer Institute), which recognizes HLA‐A, B and C.
GFAP = glial fibrillary acidic protein; HLA = human leukocyte antigen; MHC = major histocompatibility complex; TLR = toll‐like receptor.