. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: Genes Immun. 2012 Sep 20;13(8):605–620. doi: 10.1038/gene.2012.39

Table 7.

PAR-1 inhibitor treatment of infected THP-1 cells down-regulates MCP-1, MMP-1 and MMP-9 protein secretion, but does not modify M. tuberculosis intra-cellular proliferation

Culture condition/measurement	M. tuberculosis- infected, untreated	M. tuberculosis- infected, treated	Two-sample t-test
MCP-1 (pg/ml ± SD)	136 ± 39.7	0	p = 0.004
MMP-1 (pg/ml ± SD)	744.4 ± 103.4	0	p < 0.00001
MMP-9 (pg/ml ± SD)	951 ± 23	0	p < 0.0001
CFU/ml (10³)	9 ± 2.65	8.3 ± 3.1	ns

Soluble factors were determined in supernatants using ELISA test for MCP-1 (BD Biosciences) and functional specific-peptide-cleavage ELISA tests for MMP-1 and MMP-9 (R&D). Cell pellets lysed in Triton X-100 were used to recover intracellular bacteria and processed as indicated in material and methods to obtain the colony forming units (CFU) counts. SD = standard deviation; ns = not significant. The values for uninfected untreated negative control THP-1 cells were cero for MCP-1, MMP-1, and MMP-9.