Table 1.
Primer sets for PCR and PCR-RFLP used for genotyping SNPs detected in bovine MYH 3 gene
| SNPS | Amplified region | Primer sequence (5′–3′) | AT(°C) | SAF (bp) | PR | Genotype |
|---|---|---|---|---|---|---|
| SNP1 | Exon5 | AAAGCCCGAGTATCAGAACC TCCAACAGCCTCATCAAACA | 57.4 | 435 | RSaI | CC: 228, 207 |
| CT:228, 207, 150, 78 | ||||||
| TT:207, 150, 78 | ||||||
| SNP2 | Intron5 | AAAGCCCGAGTATCAGAACC TCCAACAGCCTCATCAAACA | 57.4 | 435 | BstXI | TT:435 |
| TC:435, 339, 96 | ||||||
| CC: 339, 96 | ||||||
| SNP3 | Intron7 | TTGCTGCAAATGGCATTATT TGAGATTCCCAACAAAGAGG | 59.8 | 447 | PVUII | GG:447 |
| GC:447, 222, 225 | ||||||
| CC: 222, 225 | ||||||
| SNP4 | Exon14 | TGGAGGATTTTCAGAGGGGT TGATATGGGGTGACAAGTGG | 59.3 | 532 | RSaI | CC:532 |
| CT:532, 364, 168 | ||||||
| TT:364, 168 | ||||||
| SNP5 | Exon14 | TGGAGGATTTTCAGAGGGGT TGATATGGGGTGACAAGTGG | 59.3 | 532 | TaqI | CC:433, 99 |
| CT:433, 243, 190, 99 | ||||||
| TT:243, 190, 99 |
SNPs: single nucleotide polymorphisms; SNP1 = C1878T; SNP2 = T2010C; SNP3 = G3746C; SNP4 = C7294T; SNP5 = C7315T
AT: annealing temperature, SAF: size of amplification fragment, PR: PCR-RFLP