Table 1. Phylogenetic origin of 62 plastid-localized enzymes involved in amino acid biosynthesis.

Amino acid biosynthesis pathway	Plastid-localized isoenzymes	Cyanobacterial	Non-cyanobacterial	ML bootstrap (%)
Glutamine
Glutamine synthetase	1		1	100
Glutamate
Ferredoxin-dependent Glutamate synthase (GOGAT)	2	2		>63
Tryptophan
Anthranilate synthase alpha subunit	2		2	>70
Anthranilate synthase beta subunit	2	2		<50
Anthranilate phosphoribosyl transferase	1		1	<50
Phosphoribosyl anthranilate isomerase	3		3	95
Tryptophan synthase apha subunit	2	2		>75
Tryptophan synthase beta subunit	2	2		100
Phenylalanine/Tyrosine
Arogenate dehydratase/prephenate dehydratase	6		6	<50
Arogenate dehydrogenase	2		2	<50
Histidine
ATP phosphoribosyltransferase	2		2	<50
BBMII isomerase	1		1	<50
Imidazoleglycerol-phosphate dehydratase	2	2		>94
Histidinol-phosphate aminotransferase	2		2	>95
Histidinol phosphate phosphatase	1		1	<50
Aspartate-derived
Aspartate kinase	1		1	<50
Lysine
L-diaminopimelate aminotransferase	1		1	<50
Diaminopimelate epimerase	1	1		58
Diaminopimelate decarboxylase	2		2	>94
Threonine
Threonine synthase	1		1	<50
Methionine
Cystathionine gamma-synthase	1		1	100
Isoleucine and Valine
Acetolactate synthase large subunit	1		1	99
Acetolactate synthase small subunit	1	1		95
Ketolacid reductoisomerase	1		1	<50
Leucine
Isopropylmalate isomerase	4		4	<50
Isopropylmalate dehydrogenase	4	4		>75
Arginine
N-Acetylglutamate synthase	1		1	100
N-Acetylglutamate kinase	1	1		69
N-Acetylglutamate-5-P reductase	1		1	91
N2-Acetylornithine:glutamate acetyltransferase	1	1		99
Carbamoyl-P synthetase Large subunit	1	1		100
Ornithine carbamoyltransferase	1		1	<50
P II nitrogen sensing protein GLB I	1	1		<50
Serine
3-phosphoglycerate dehydrogenase	2		2	<50
Glycine
Serine (glycine) hydroxymethyltransferase	2		2	65
Serine (alanine):glyoxylate aminotransferase	1		1	94
Alanine
Cysteine desulfurase	1	1		<50
Total	62	21	33+8