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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Eur J Cancer. 2012 Jun 2;48(18):3488–3498. doi: 10.1016/j.ejca.2012.04.020

Figure 4. Effects of the c-Src inhibitor on ER negative cell lines.

Figure 4

4A. Blocking c-Src phosphorylation in ER negative cell lines by PP2. ER negative cells were treated with PP2 (5μM) for different times as indicated and cell lysates were harvested. Phosphorylated c-Src was detected by immunoblotting with primary antibody. Immunoblotting for total c-Src was used for loading control. 4B. Inhibitory effects of PP2 on ER negative cells. ER negative cells were seeded in 24-well plates in triplicate. After one day, the cells were treated with vehicle (0.1%DMSO) and PP2 (5μM) in 10% SFS medium. The cells were harvested after 7 days treatment and total DNA was determined as above. 4C. Signaling pathways were changed in ER negative cells after PP2 treatment. ER negative cells were treated with PP2 (5μM) for different times as indicated and cell lysates were harvested. Phosphorylated MAPK and Akt were examined by immunoblotting with primary antibodies. Immunoblotting for total MAPK and Akt were determined for loading controls.