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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Eur J Cancer. 2012 Jun 2;48(18):3488–3498. doi: 10.1016/j.ejca.2012.04.020

Figure 6. Blocking c-Src sensitized cell to antiestrogen in Sk-Br-3 cells.

Figure 6

6A. ERα expression was elevated in Sk-Br-3 cells after PP2 treatment. Sk-Br-3 cells were treated with PP2 as indicated. ERα expression was examined by immunoblotting with primary antibody. Immunoblotting for β-actin was determined for loading control. 6B. ERα mRNA was increased in Sk-Br-3 cells after PP2 treatment. Sk-Br-3 cells were treated with PP2 (5μM) for the times as indicated. The RNA was harvested in TRIzol for real-time PCR analysis. P<0.05, * compared with control. 6C. HER2 expression was downregualted in Sk-Br-3 cells after PP2 treatment. Sk-Br-3 cells were treated with PP2 for the times as indicated. HER2 was examined by immunoblotting with primary antibody. Immunoblotting for β-actin was determined for loading control. 6D. The PP2 sensitized Sk-Br-3 cells to 4-hydroxytamoxifen. Sk-Br-3 cells were treated with vehicle, 4-OHT (1μΜ), PP2 (5μM), and 4-OHT (1μΜ) plus PP2 (5μM) in 10% FBS medium. The cells were harvested after 7 days treatment and total DNA was determined as above. P<0.05, * compared with control.