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. Author manuscript; available in PMC: 2012 Dec 11.
Published in final edited form as: Nat Commun. 2010 Aug 10;1:54. doi: 10.1038/ncomms1054

Figure 2.

Figure 2

Single molecule content mixing assay for yeast SNARE-mediated fusion. (a) Schematics of the assay. Vesicles reconstituted with Snc2p proteins (v-vesicles) and encapsulating dual labeled DNA probes are immobilized on the surface of the flow cell. Vesicles reconstituted with Sso1pHT proteins (t-vesicles) and encapsulating poly-A DNA strands are flown in along with soluble Sec9c proteins, and the sample is incubated at 37 °C. (b) E distributions of v-vesicles before the reaction, and (c) after incubating for 30 min with t-vesicles and 1 μM Sec9c protein.