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. Author manuscript; available in PMC: 2012 Dec 11.
Published in final edited form as: Nat Commun. 2010 Aug 10;1:54. doi: 10.1038/ncomms1054

Figure 4.

Figure 4

Content mixing requires full SNARE complex with the wild type Sec9c. (a) E distributions of fusion products for different Sec9c concentrations. (b) E distributions of yeast SNARE-mediated fusion with different incubation times ranging from 0 min to 30 min with 1 μM Sec9c. (c) Pore expansion efficiency quantified by low FRET peak percentage (E = 0-0.4) for wild type and mutant Sec9c, L626P and L646P. 1 μM Sec9c and its mutants were used. Error bars denote the standard deviation of three independent experiments. 30 min incubation at 37 °C was applied for all experiments in (a) and (c).