Table 1.
Bacterial strains and plasmids used in this study.
| Strain | Relevant properties | Source or references |
|---|---|---|
| Y. pestis | ||
| KIM5 | Pgm− Lcr+; pCD1 pMT1 pPCP1a; conditionally virulent Δpgm 2.MED strain; also called KIM D27; YopM+ parent strain | Brubaker, 1991 |
| KIM5-3002 | ΔyopM-1 derivative of Y. pestis KIM5 | Kerschen et al., 2004 |
| KIM8-3003.12 | pPCP1− Multiple-Yop mutant; ΔyopM-1 | Philipovskiy et al., 2005 |
| (KIM8-MYM) | ΔyopH ΔyopE ΔyopT ΔypkA ΔyopJ | |
| E. coli | ||
| DH5α | F− ΔΦ80d lacZΔM15 endA1 recA1 hsdR17(r−m m+k) supE44 thi-1 gyrA96 Δ (lacZYA-argF)U169; cloning host | Life technologies |
| PLASMIDS | ||
| pBluescript II SK- | Phagemid cloning vector | Stratagene (Agilent) |
| pBS10 (pYopM) | yopM and native promoter in pBluescript II SK- | Reisner and Straley, 1992 |
| pPCP2::Kan | kan-marked pPCP2 (from Y. pestis CO92) | Forman et al., 2008 |
a
The native virulence plasmids of Y. pestis are the 9.6 kb pPCP (encodes the protease Pla), the 70.3 kb Lcr plasmid pCD (encodes the Ysc T3SS and Yops), and the 96.2 kb pFra (also called pMT; encodes the capsular fibril F1) (Perry and Fetherston, 1997).