Table 4.
Cytokine/chemokine concentration, pg ml−1 | ||||||||
---|---|---|---|---|---|---|---|---|
Treatment | G-CSF | GM-CSF | TNFα | IL-1β | IL-6 | CXCL1 | IFNγ | |
Sera | ||||||||
Non-infected mice | 452 ± 93 | <160 | 10 ± 1 | <160 | <32 | 74 ± 18 | 20 ± 8 | |
Mice infected 16 h | ||||||||
Parent Y. pestis | 97,401 ± 27,167 | <160 | 43 ± 5 | <160 | 1341 ± 485 | 2087 ± 231 | 7291 ± 3042 | |
ΔyopM-1 Y. pestis | 111,939 ± 42,016 | <160 | 48 ± 5 | <160 | 2476 ± 413 | 2351 ± 869 | 6876 ± 1326 | |
CD11b+ CELLS | ||||||||
Non-infected mice | <3 | <16 | 38 ± 19 | <16 | 33 ± 3 | 7 ± 2 | <3 | |
Mice infected 1 h | ||||||||
Parent Y. pestis | 10 ± 3c | <16 | 149 ± 139c | <16 | –b | 19 ± 14c | <3 | |
ΔyopM-1 Y. pestis | –b | <16 | 53 ± 65 | <16 | –b | 8 ± 4c | <3 | |
Mice infected 16 h | ||||||||
Parent Y. pestis | –b | 22 ± 2c | 27 ± 27 | 48 ± 22 | 150 ± 155 | 23 ± 12 | 1712 ± 1040 | |
ΔyopM-1 Y. pestis | –b | <16 | 373 ± 519 | 48 ± 16* | 1076 ± 1478 | 41 ± 25 | 650 ± 249* | |
CCL5 | CXCL9 | CXCL10 | CXCL2 | VEGF | IL-10 | IL-17 | ||
SERA | ||||||||
Non-infected Mice | 54 ± 4 | 410 ± 53 | 220 ± 14 | <800 | <6 | <32 | 8 ± 0 | |
Mice infected 16 h | ||||||||
Parent Y. pestis | 80 ± 35 | 76,612 ± 3276 | 3116 ± 186 | 1151 ± 281c | 28 ± 10 | –b | 11 ± 6 | |
ΔyopM-1 Y. pestis | 88 ± 16 | 50,585 ± 14,345 | 3709 ± 215** | 969 ± 86c | 23 ± 0c | <32 | 15 ± 2 | |
CD11b+ CELLS | ||||||||
Non-infected mice | 123 ± 73 | 335 ± 256 | 391 ± 225 | –b | <3 | –b | <3 | |
Mice infected 1 h | ||||||||
Parent Y. pestis | 49 ± 25 | 168 ± 63 | 235 ± 156 | –b | <3 | <3 | <3 | |
Δ yopM-1 Y. pestis | 35 ± 8 | 117 ± 14 | 217 ± 85 | –b | <3 | <3 | <3 | |
Mice infected 16 h | ||||||||
Parent Y. pestis | 31 ± 16 | 1609 ± 868 | 1112 ± 660 | <80 | <3 | <3 | –b | |
ΔyopM-1 Y. pestis | 74 ± 82 | 4373 ± 2751 | 2127 ± 1521 | –b | <3 | –b | 7 ± 4c | |
IL-4 | IL-12(p40) | IL-12(p70) | IL-15 | Eotaxin | CXCL5 | CCL2 | M-CSF | |
SERA | ||||||||
Non-infected mice | 9 ± 1c | <32 | 139 ± 32 | <32 | 568 ± 47 | 7994 ± 631 | 76 ± 5 | 8 ± 2 |
Parent Y. pestis | –b | 86 ± 28 | 267 ± 142 | 942 ± 1188 | 1584 ± 47 | 7660 ± 450 | 2047 ± 237 | 44 ± 10c |
ΔyopM-1 Y. pestis | –b | 81 ± 13c | 262 ± 19 | 54 ± 19c | 1537 ± 154 | 7756 ± 536 | 1804 ± 245 | 37 ± 4 |
CD11b+ cellsd | <3 | <16 | <3 | <16 | <3 | <400 | <16 | <3 |
<signifies that data from all three experiments were below the detection limit. Other values without a superscript are average ± SD based on data that were above the detection limit in all three experiments.
–denotes that there was only one response above the detection limit; the other two experiments returned data that were below the detection limit.
Average ± the range from two experiments; the third experiment returned data that were below the detection limit.
CD11b+ cells from all treatment groups returned responses below the detection limit for this set of genes, with the exception of mice infected for 16 h with parent Y. pestis, for which a single experiment returned a value of 670 pg ml−1 for CXCL5.
Significantly greater than the response from CD11b+ cells from non-infected mice (P < 0.05).
Data from mice infected with the two Y. pestis strains differed significantly at P < 0.05.