Table 2.

96 well plate map for screening of generic peptide probes against disease state 1 and disease state 2 plasma samples from two different patients

		Patient 1						Patient 2
		Disease State 1 Plasma			Disease State 2 Plasma			Disease State 1 Plasma			Disease State 2 Plasma
		Pep 1	Pep 2	Pep 3	Pep 1	Pep 2	Pep 3	Pep 1	Pep 2	Pep 3	Pep 1	Pep 2	Pep 3
	Row/Column	1	2	3	4	5	6	7	8	9	10	11	12
Buffer 1-HEPES pH 8 - Serine Protease	A
	B
Buffer 2-Tris pH 7.5 - Metallo Protease	C
	D
Buffer 3-Citrate pH 5.5 - Cysteine Protease	E
	F
Buffer 4 -Citrate pH 4.0 - Aspartyl Protease	G
	H

A sample 96 well plate for screening of three generic peptide probes against plasma samples collected from two different patients representing different disease states. Peptide 1, 2 and 3 corresponds to the generic peptide probes Mca-RPPGFSAFK (Dnp), CathD and E substrate, and Amyloid precursor protein (peptide sequences listed in Table 1) respectively. The final concentration of the peptides in each reaction well is 62.5 μM and final dilution of all the plasma samples was 1:4. The ‘Experimental plate’ is generated by pipetting respective volumes of buffer and peptides in a 96 well plate as per the plate map mentioned above. The ‘Sample plate’ is prepared in a fresh 96 well plate by pipetting 25 μL of 1:1 dilution of plasma sample as per the plate map defined above. Using an 8-channel pipettor 20 μL of plasma from each of the 8 wells in the column of the ‘Sample plate’ is transferred to the corresponding column in the ‘Experimental plate’.