Table 1.
Parasitological data of BALB/c mice vaccinated with recombinant first extracellular domain of Schistosoma mansoni tetraspanning orphan receptor (rSmTORed1), inclusion bodies (i. bodies) or phosphate-buffered saline (PBS) all adjuvanted with complete Freund's adjuvant/incomplete Freund's adjuvant (CFA/IFA)
| Mean worm burden ± s.d. % reduction, P-value | Total worm burden Mean ± s.d. % reduction, P-value | Number of eggs per gram liver tissue; epg × 103 Mean ± s.d. % reduction, P-value | ||||
|---|---|---|---|---|---|---|
| Adult worms, range | Male | Female | Pairs | |||
| Trial 1 | ||||||
| Control (PBS) n = 10 | 12–53 | 16·5 ± 8·3 | 12·3 ± 6·7 | 8·7 ± 6 | 28·8 ± 14·6 | ND |
| Control (i. bodies) n = 10 | 4–51 | 14·1 ± 9·8 | 10·1 ± 7·7 | 9·5 ± 7·9 | 24·2 ± 17·4 | ND |
| rSmTORed1 n = 10 | 1–31 | 5·7 ± 4·7 | 4·7 ± 4·2 | 2·9 ± 2·3 | 10·4 ± 8·6 | ND |
| 651/602 % | 621/532 % | 671/692 % | 641/572 % | |||
| P = 0·0131 | P = 0·0038 | P = 0·010 | P = 0·0058 | |||
| Trial 2 | ||||||
| Control (PBS) n = 8 | 12–44 | 10·3 ± 4·7 | 11·3 ± 5·9 | 6·5 ± 4 | 21·5 ± 10·5 | 11·75 ± 5·19 |
| rSmTORed1 n = 10 | 5–19 | 5·5 ± 4·2 | 6·4 ± 3·2 | 4 ± 2·5 | 11·9 ± 5·2 | 5·88 ± 2·99 |
| 47%, P = 0·0138 | 43%, P = 0·0393 | 38%, P = 0·125 | 45%, P = 0·0212 | 50%, P = 0·0082 | ||
Statistical analyses were performed using Student's t-test. P-values are shown for comparisons between the immunized versus both groups of controls taken together (trial 1) and immunized versus PBS/CFA injected mice (trial 2). n: the number of mice per group (from a total of 10) that were included for analysis. Comparison of numbers of parasites was between animals immunized with rSmTORed1 and the correspondent control groups, PBS-injected animals1 (comparison 1) and animals injected with inclusion bodies2 (comparison 2). ND: not determined; i. bodies: inclusion bodies; s.d.: standard deviation.