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. 2012 Dec 3;2012:956875. doi: 10.1155/2012/956875

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Copyright © 2012 Laura Mónica Álvarez-Rodríguez et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Obtaining recombinant NS3 protein. (a) Cloning strategy for the NS3 domain protease of dengue virus in the cloning vector and in prokaryotic expression vector. (b) Amplification of the NS3 protease gene cloned into pCR2.1TOPO, (M) size marker, Lane 1, NS3-DEN1. Lane 2, NS3-DEN2. Lane 3, NS3-DEN3. Lane 4, NS3-DEN4. (c) Purification of the recombinant protein GSTNS3 of each serotype. (M) molecular weight marker. Lane 1, GST-NS3DEN1. Lane 2, GST-NS3DEN2. Lane 3, GST-NS3DEN3. Lane 4, GST-NS3DEN4. Lane 5, GST.