Figure 7.

Long 3′ UTR Bdnf mRNA facilitates neuronal integration. A, Representative confocal images of activity-dependent Arc expression in adult-born cells. Mice were treated with BrdU at 4 weeks of age. Four weeks post-BrdU injection, kainate (35 mg/kg, i.p.) was used to induce grade 4–5 seizures. Two hours postseizure induction, animals were killed for immunohistochemistry. Scale bar, 50 μm. B, Percentage of BrdU⁺ cells that also expressed Arc 4 weeks post-BrdU injection and 2 h postseizure induction (n = 3 mice for +/+ and n = 4 mice for k/k). Error bars indicate SEs. C, Representative confocal images of colocalization of BrdU with Zif268 in the dentate gyrus of +/+ and k/k mice. Mice were treated as described in A. Scale bar, 50 μm. D, Percentage of BrdU⁺ cells that expressed Zif268 4 weeks post-BrdU injection and 2 h postseizure induction (n = 3 mice for +/+ and n = 4 mice for k/k). Error bars indicate SEs. E, Representative images of Arc immunohistochemistry in the dentate gyrus of +/+ and k/k. Mice were treated as described in A. Scale bar, 50 μm. F, Density of Arc⁺ cells in the dentate gyrus of +/+ and k/k mice. Error bars indicate SEs. G, Representative images of Zif268 immunohistochemistry in the dentate gyrus of +/+ and k/k mice. Mice were treated as described in A. Scale bar, 50 μm. H, Density of Zif268⁺ cells in the dentate gyrus of +/+ and k/k mice. Error bars indicate SEs. Student's t test: **p < 0.01.