Figure 4.

Overexpression of Cxcr4 in bone marrow derived stem cells. The cells at passage 5 were transfected with a mouse Cxcr4 expression vector (pCMV6-Cxcr4) and the stable cell clones expressing Cxcr4 were established. RT-PCR was conducted to examine the mRNA levels of mouse Crcx4 in the transfected or untransfected stem cells at the same passage. Lane 0: 100 bp DNA ladder; lane 1: reverse transcribed sample from the transfected cells; lane 2: reverse transcribed sample from the untransfected cells; lane 3: nonreverse transcribed sample from the transfected cells; lane 4: nonreverse transcribed sample from the untransfected cells; lane 5: H₂O blank.