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. Author manuscript; available in PMC: 2013 Nov 20.
Published in final edited form as: Biochemistry. 2012 Nov 7;51(46):9394–9405. doi: 10.1021/bi301176c

Figure 6. The ANF-RGC activity in mouse adrenal gland is Ca2+-dependent.

Figure 6

Particulate fractions of mouse adrenal gland were isolated in the presence of 1 mM EGTA (panel “−Ca2+”) or 10 μM Ca2+ (panel “+Ca2+”) as described in the “Experimental Procedures” section. The membranes were assayed for guanylate cyclase activity in the presence of 1 mM EGTA or 1 μM Ca2+. The experiment was repeated three times with separate membrane preparations. The results are mean ± SD of these experiments.