Figure 8. Neurocalcin δ modulates ANF-RGC activity in mouse adrenal gland.

(A) Adrenal glands were removed from the wild type (WT) and neurocalcin δ^+/− (NCδ^+/−) mice and their particulate fractions were isolated in the presence of 1 mM EGTA (panels “−Ca²⁺” in “WT membranes” and “NCδ^+/− membranes” sections of the figure) or 10 μM Ca²⁺ (panels “+Ca²⁺” in both sections of the figure). These were assayed for guanylate cyclase activity in the presence of 1 mM EGTA or 1 μM Ca²⁺. (B) 2 μM myr-NCδ was added to the adrenal gland membranes isolated in the presence of 10 μM Ca²⁺ from the wild type (“WT memb.”) or NCδ^+/− (“NCδ^+/− memb.”) mice and the guanylate cyclase activity was assessed in the presence of 1 μM Ca²⁺. The experiments were repeated two times with separate membrane preparations. The results are mean ± SD of these experiments.