Table 4. Analysis of CDHR1 intron 13 donor splice-site and adjacent potential sites by the Automated Splice Site Analyses tool.

Genomic coordinate*	wt sequence	Mutant sequence	Position relative to natural site	Initial score (Ri)†	Final score (Ri)‡	ΔRi║
85,970,922–85970923	acagtggg	acaggggg	0	4.0	−4.2	−8.2
85,970,950–85970951	tgggtggg	NA	+27	3.8	3.8	0.0

NA, not applicable *Position of donor site according to human genome reference sequence (hg19) †Initial score measured at the base before the mutation is made ‡ Final score measured at the base after the mutation is made ║Final (Ri)-Initial (Ri)