Table 4. Analysis of CDHR1 intron 13 donor splice-site and adjacent potential sites by the Automated Splice Site Analyses tool.
Genomic coordinate* | wt sequence | Mutant sequence | Position relative to natural site | Initial score (Ri)† | Final score (Ri)‡ | ΔRi║ |
---|---|---|---|---|---|---|
85,970,922–85970923 |
acagtggg |
acaggggg |
0 |
4.0 |
−4.2 |
−8.2 |
85,970,950–85970951 | tgggtggg | NA | +27 | 3.8 | 3.8 | 0.0 |
NA, not applicable *Position of donor site according to human genome reference sequence (hg19) †Initial score measured at the base before the mutation is made ‡ Final score measured at the base after the mutation is made ║Final (Ri)-Initial (Ri)