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Immunopanning-magnetic separation method for isolating retinal ganglion cells. Retinal cell suspension is incubated with antimacrophage antibody and distributed over an antiimmunoglobulin G antibody-coated Petri dish. Non-adherent cells are treated with biotinylated anti-Thy 1 antibody and subsequently interacted with antibiotin magnetic MicroBeads. Finally, the magnetic-labeled retinal ganglion cells were collected using a column placed in the magnetic field.
