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. 2012 Sep 5;32(12):2171–2180. doi: 10.1038/jcbfm.2012.123

Figure 2.

Figure 2

Total NOTCH3 and α-SMA expression in CADASIL and control vascular smooth muscle cells (VSMCs). (A) Western blot analysis of the expression of NOTCH3 detected with 5E1 directed against N3ECD in CADASIL and control VSMCs. In all, 40 μg of cell lysate was loaded on each lane. The 280-kDa full-length NOTCH3 (N3FL) and 220 kDa N3ECD are indicated with arrowheads. The 245-kDa band in between (white arrowhead) is unknown. (B) The expression of NOTCH3 is significantly higher in HCerVSMCs and HArtVSMCs than in HUmbVSMCs or HPlaVSMCs. There are no statistically significant differences in the total expression level of NOTCH3 or N3FL/N3ECD ratio between CADASIL versus the corresponding control or between control versus heterozygous or homozygous VSMCs. (C) Western blot analysis of α-SMA expression in CADASIL and control VSMCs. In all, 20 μg of cell lysate was loaded on each lane. (D) Even though, CADASIL cells have altered actin filament organization, the total amount of α-SMA in CADASIL VSMCs is not significantly different from that in corresponding control VSMCs. The bar graphs show the average intensities and ±s.e.m. from two independent experiments. Both experiments were repeated two times with cells at passage 5.