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. 2012 Sep 5;32(12):2171–2180. doi: 10.1038/jcbfm.2012.123

Figure 5.

Figure 5

Integrin β1 and adapter protein tensin in adhesion sites. Methanol fixed vascular smooth muscle cells (VSMCs) were immunostained with integrin β1 or tensin antibody. (A–F) Integrin β1 forms thin ribbon-like structures in the center of the cells, parts of which are vinculin positive. In the two CADASIL (only HUmbVSMC shown) VSMC lines analyzed the β1 positive ribbons were more frequent than in the two control VSMC lines (only HUmbVSMC shown). (G–L) Tensin colocalizes partly to the same adhesion structures as vinculin in both CADASIL VSMC lines analyzed and control cell lines (only HUmbVSMC shown). (M) Bar graph shows the average volume (area × intensity) of tensin immunoreactivity in CADASIL and control VSMCs (P<0.01). From each cell line, at least 500 adhesion sites were measured. Tensin localization indicates maturation of adhesion sites to the stages of late focal adhesions and fibrillar adhesions and the change from a structural adhesion to an adhesion that interacts with signaling.