Table 1. VSMC lines used in this study.

Cell line	Origin	Genotypes (n)	Phenotype	Patient age
HUmbVSMC	Post partum, umbilical cord artery	wt (4) and p.R133C (4)	Spindle-shaped	Newborn
HPlaVSMC	Post partum, placental vessel	wt (4) and p.R133C (4)	Spindle-shaped	Newborn
HArtVSMC	Surgery and post mortem, Adult systemic artery	p.R133C (3)	Spindle-shaped	p.R133C: 61, 66, 64
HCerVSMC	Post mortem, subarachnoidal branches of cerebral arteries	wt (3) p.R133C (2); p.R133C-hmz (1) p.G528C (1)	Epithelioid-shaped	WT: 61, 73, 82 p.R133C: 64, 65 p.R133C-hmz: 62 p.G528C: 68

Cell line designates the abbreviated names of the cell lines used in the text. Origin defines the vessel from which the cell line has been isolated. Genotype shows the NOTCH3 mutation detected in the cell lines. VSMC=vascular smooth muscle cell, wt=wild type, n=number of cell lines analyzed. Phenotype designates the observed phenotype under phase contrast light microscopy. Age designates the age of the patient from whom the cell line was established.