Table 1.
Gene | Forward primer | Reverse primer | Enzyme | Annealing |
---|---|---|---|---|
MTHFR 677 | CTTTGGGGAGCTGAAGGACTACTAC | CACTTTGTGACCCCG GTTTG | Hinf I | 62° |
MTHFR 1298 | CTTTGGGGAGCTGAAGGACTACTAC | CACTTTGTGACCCCGGTTTG | Mbo II | 60° |
TS 3′ UTR | CAAATCTGAGGGAGCTGAGT | CAGATAAGTGGCAGTACAGA | Dra I | 58° |
TS 5′ repeat | GTGGCTCCTGCGTTTCCCCC | GCTCCGAGCCGGCCACAGGCATGGCGCGG | Hae III | 65° |
CDA A79C | GGTACCAACATGGCCCAGAA | CCTTTGAAGATTCTCCCCTCC | n.a. | 62° |
RRMI | TTCCTTGTAGGGTTTGAAGA | AGGATCCACACATCA GACAT | n.a. | 57° |