Skip to main content
. 2012 Dec 10;7(12):e51135. doi: 10.1371/journal.pone.0051135

Figure 4. Effect of native (h/rOb) and Q90L obestatin (hObQ90L) on ghrelin-induced (Ghr) cFos immunoreactivity in C57BL/6 mice in the arcuate nucleus (ARC) and in the nucleus tractus solitarius (NTS).

Figure 4

(A and C) Representative confocal photomicrophotographs of the cFos staining after each treatment in coronal sections at 1.9 mm of the interaural line in the ARC and −3.78 mm of the interaural line in the NTS ; (B and D) Average of cFos-positive nuclei per section after the different treatments between 2.2/1.6 mm of the interaural line for the ARC and between −3.78/−3.98 mm of the interaural line for the NTS. Data represent mean±SEM. ***P<0.001 vs saline, *P<0.05 vs saline, #P<0.05 vs ghr. Scale bar represents 100 µm in the ARC and 50 µm in the NTS. 3V: third ventricle, ME: median eminence, DMH: Dorsomedial Hypothalamic nucleus, VMH: Ventromedial Hypothalamic nucleus, ARC: Arcuate nucleus, NTS: Nucleus Tractus Solitarius, AP: Area Postrema, 10N: Dorsal Motor Nucleus of Vagus, cc: central canal.