Figure 5. Effect of native (h/rOb) and Q90L obestatin (hObQ90L) on ghrelin-induced (Ghr) cFos immunoreactivity in arcuate nucleus (ARC) NPY neurons in C57BL/6 mice.

(A) Representative confocal photomicrophotographs of hypothalamic coronal sections showing the merged images containing cFos staining in red and NPY-GFP cells in green after each treatment at 1.9 mm of the interaural line in the ARC. (B) Quantification of NPY-GFP positive cell bodies expressing cFos in the ARC after the different treatments. Data represent mean±SEM. ***P<0.001 vs saline, #P<0.05 vs ghr, $P<0.01 vs ghr+hOb. Scale bar represents 100 µm, 3V: third ventricle, ME: median eminence.