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. 2012 Oct 24;12:492. doi: 10.1186/1471-2407-12-492

Figure 4.

Figure 4

Effects of functional manipulation of miR-17-5p on DHT-induced AR transcriptional activity and cell growth.A and B, impact of functional manipulation of miR-17-5p on DHT-induced transcription of the PSA gene. LNCaP cells were treated with anti-miR-17-5p or miR-17-5p precursor for 48 h and then exposed to DHT (10 nM) for an additional 24 h, followed by qRT-PCR analysis. C - F, impact of functional manipulation of miR-17-5p on DHT-induced PSA-6 kb luciferase activity in LNCaP cells (C and D) and C4-2B cells (E and F). Cells were transfected with the PSA-6 kb luciferase reporter plasmid and simultaneously treated with anti-miR-17-5p or miR-17-5p precursor for 48 h, then exposed to DHT (10 nM) for an additional 24 h. Luciferase activity was measured and presented as the ratio to β-gal. G, inhibition of miR-17-5p precursor on DHT-stimulated LNCaP cell growth. Cells were treated with miR-17-5p precursor for 48 h then exposed to DHT (10 nM) for 72 h, followed by MTS assay. Data are averages of three independent experiments. *, p < 0.05 compared to non-DHT-treated cells (in A -G); #, p < 0.05 compared to cells treated with DHT only. Luc = luciferase activity.