Figure 6. Resveratrol downregulates LMP1 expression in EBV-infected B cells.

(A) EBV-infected B cells were cultured for up to 96 hours with the indicated concentrations of resveratrol. Cellular RNA was extracted and the levels of LMP1 transcripts were determined using qRT-PCR. Error bars represent means±SEM of three independent experiments (B) B cells were infected with EBV for 72 hours and then cultured in the presence or absence of resveratrol for another 48 hours, after which the expression of LMP1 proteins was assessed using Western blotting. (C) Two EBV-immortalized LCLs were cultured with or without resveratrol for 48 hours and the levels of LMP1 transcripts were assessed using RT-PCR. Error bars represent means±SEM of three independent experiments (D) Whole-cell proteins from LCLs that were treated as in panel C were subjected to Western blotting and probed with anti-LMP1 antibodies. A representative figure of three independent experiments is shown. LCLs were treated as in panel C and the whole-cell protein extracts were analyzed using Western blotting with antibodies specific for EBNA1 (E) and EBNA-2 (F). The figures shown are representative results of three independent experiments.