Skip to main content
. 2012 Dec 10;7(12):e51397. doi: 10.1371/journal.pone.0051397

Figure 3. The fine-specificity of an Env183/A2 mAb and soluble TCR tetramers.

Figure 3

(A) The Env183–191 peptide modeled into the Tax/HLA-A2 crystal structure, inset: lateral view of the peptide in HLA-A*02:01. The Arg187 side chain protrudes from the binding pocket, potentially interacting with the TCR or antibody. (B) Nine different Env183–191 peptides with single position alanine substitutions were used to probe the fine specificity. (C and D) The alanine variant pMHCs were loaded onto beads and stained with Env183/A2 mAb or TCR tetramer (1 µg/mL).