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. 2012 May 24;12:123. doi: 10.1186/1471-2334-12-123

Figure 3.

Figure 3

Analysis of intracytoplasmic cytokine profile of CD8+. T-lymphocytes in the peripheral blood from untreated Indeterminate chagasic patients (IND gray rectangle), Bz-treated Indeterminate chagasic patients (INDt black rectangle) as compared to non-infected individual (NI empty rectangle). Data are expressed as median absolute counts plus the interquartile range of cytokine+ cells observed after short-term in vitro “Control Culture” (top panel) and T. cruzi epimastigote antigen “Stimulated Culture” (bottom panel) of whole-blood samples. Cytokine+ CD8+ T-cells were quantified by dual color immunophenotyping (anti-CD8-TC plus anti-cytokine-PE mAbs) using specific gate strategy, as described in Material and Methods. The significant differences at p < 0.05 for comparisons with NI are indicated by the letter ‘a’. The significant differences at p < 0.05 for comparisons with INDt are indicated by connecting lines. The significant differences at p < 0.05 for comparative analysis between “Control” and “Stimulated” cultures are indicated by *.