Figure 6. Diseased TAK1^ΔM mice developed myelomonocytic leukemia.

(A) Peripheral blood smears (left panels) and bone marrow smears (right panels) from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with Wright-Giemsa (WG) and H&E, respectively. Note numerous monocytes, some with azurophilic granules (arrows) in the peripheral blood and increased blasts in bone marrow aspirate smears of TAK1^ΔM mice. Also shown for the BM smear is the percentage of a manual count of blasts and monocytes (Mo). (B) Blood and bone marrow smears from diseased TAK1^ΔM mice were stained with Wright-Giemsa (WG) and non-specific esterase (NSE). Note the increased number of blasts in the peripheral blood film and sheets of blasts in the bone marrow aspirate smear (arrows). A subset of blasts was positive for NSE (inset and lower panel). (C) Bone marrow biopsies from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with H&E. Note the increased number of immature cells (dotted circle) in the TAK1^ΔM mice. Magnification: left panels, 200x; right panels, 400x. (D–E) Tissue sections of spleens from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with H&E (D) and NASD (E, left panel) and immunostained with an anti-Mac-3 antibody (E, right panel).