Table 1.

Sequences of the primers used in this study

Peptides	Primer sequences (5’→3’)	Position	Size of amplicon
tM-His6	F: ATACATATGAGCATTCGGTTGTGGCG	295–678	384bp
	R: CGCCTCGAGGACTAAATGAAGCACT
	F: TAAGGATCCAGCATTCGGTTGTGGCGCAG
GST-tM	R: CGGCTCGAGGACTAAATGAAGCACTTTCTCAC	295–678	384bp
	F: TATCATATGAGCATTCGGTTGTGG
M1	R: TATCTCGAGTACGCCAGTAGCAAC	295–480	186bp
	F: GCGCATATGCTCACTACTTCTGTGAT
M2	R: GCGCTCGAGATAGACAATTGTTGTAG	358–540	183bp
	F: GCACATATGGTACAGGTAAGTCAAT
M3	R: CTACTCGAGGACTAAATGAAGCACT	478–678	201bp
	F: gatccGTACAGGTAAGTCAATTACCTAATTTCGTCACAGTCGCCA
M4	AGGCCACTACAACAATTGTCTATGGACGTGTTtaac	478-549	72bp
	R: tcgagttaAACACGTCCATAGACAATTGTTGTAGTGGCCTTGGCGA
	CTGTGACGAAATTAGGTAATTGACTTACCTGTACg
	F: gatccGGACGTGTTGGTCGTTCAGTCAATGCTTCATCTGGCACTG
M5	GTTGGGCTTTCTATGTCCGGtaac	541–600	60bp
	R: tcgagttaCCGGACATAGAAAGCCCAACCAGTGCCAGATGAAGCA
	TTGACTGAACGACCAACACGTCCg
M6	F: gatccTCAGCTGTGAGTAATCCGAGTGCGGTTCTCACAGATtaac	592–627	36bp
	R: tcgagttaCACAGCTGAGTAGTCGCCGTGTTTTGACCGGACATAg
M7	F: gatccTCAGCTGTGAGTAATCCGAGTGCGGTTCTCACAGATtaac	619–654	36bp
	R: tcgagttaATCTGTGAGAACCGCACTCGGATTACTCACAGCTGAg F: gatccCTCACAGATAGTGAGAAAGTGCTTCATTTAGTCtaac
M8	R: tcgagttaGACTAAATGAAGCACTTTCTCACTATCTGTGAGg	646–678	33bp
M9	F: gatccGGACGTGTTGGTCGTTCAGTCAATtaac	541–564	24bp
	R: tcgagttaATTGACTGAACGACCAACACGTCCg
M10	F: gatccCGTTCAGTCAATGCTTCATCTGGCtaac	553–576	24bp
	R: tcgagttaATTGACTGAACGACCAACACGTCCg
M11	F: gatccGCTTCATCTGGCACTGGTTGGGCTtaac	565–588	24bp
	R: tcgagttaAGCCCAACCAGTGCCAGATGAAGCg
M12	F: gatccACTGGTTGGGCTTTCTATGTCCGGtaac	577–600	24bp
	R: tcgagttaCCGGACATAGAAAGCCCAACCAGTg
M13	F: gatccGGTTGGGCTTTCTATGTCCGGtaac	580–600	21bp
	R: tcgagttaCCGGACATAGAAAGCCCAACCg
M14	F: gatccTGGGCTTTCTATGTCCGGtaac	583–600	18bp
	R: tcgagttaCCGGACATAGAAAGCCCAg
	F003A gatccGCTTTCTATGTCCGGtaac
M15	R: tcgagttaCCGGACATAGAAAGCg	586–600	15bp
	R: tcgagttaGACATAGAAAGCCCAg
M16	F: gatccTGGGCTTTCTATtaac	583–597	15bp
	R: tcgagttaGACATAGAAAGCCCAg
M17	F: gatccTGGGCTTTCTATtaac	583–594	12bp
	R: tcgagttaATAGAAAGCCCAg

The introduced restriction enzyme sites (NdeI and XhoI or BamHI and XhoI) are underlined. At the 5’ and 3’ terminal of each forward strand there is a sequence “gatcc” and “taac” (in italics), respectively. At the 3’ and 5’ terminal of each reverse strand there is a sequence “g” and “tcgagtta” (in italics), respectively. When the forward and reverse oligonucleotides annealed they form a cohesive BamHI site or NdeI at the 5’ terminus and a cohesive XhoI site at the 3’ terminus. The bases “taa” and “tta” (in bold) were introduced into each pair of oligonucleotides to form a stop codon.