Figure 1. Drosophila oogenesis. Egg chambers from a subset of the stages of oogenesis, stained with phalloidin to visualize F-actin (red) and DAPI to visualize DNA (blue), reveal the major changes in the size and shape of the egg chamber and its component cells over time. A single focal plane through the center of each chamber is shown. Dotted lines separate egg chambers that were obtained from distinct ovarioles. A higher magnification image of the stage 7 egg chamber is included to show how the cuboidal follicle cell layer completely surrounds the germline cells at this stage. A similar organization of the egg chamber is seen from stages 1–8. The anterior/posterior (A/P) and dorsal/ventral (D/V) axes are indicated. Ger, germarium; St, stage.