Figure 6. Loss of the round egg gene Lar results in follicle cell basal actin filaments that remain parallel within each cell but are no longer uniformly oriented across the follicle cell layer. High magnification views of a subset of follicle cells from a stage 12 wild-type egg chamber (A) or a stage 12 egg chamber that contains a clone of Lar mutant follicle cells (B). Both egg chambers have been stained with phalloidin to visualize F-actin (red) and an antibody against βPS integrin (green). Inset in (B) shows GFP marking wild-type cells at one-third scale. The mean orientation of actin filaments within wild-type cells in (B) is indicated with arrows. While some wild-type cells retain the proper orientation (far left cells), those closest to the mutant clone display defects in filament orientation, which resemble those in the Lar mutant cells (far right, without arrows). Orientation of the A/P axis of the egg chamber is indicated. (A and B) reprinted from reference 29 with permission from Elsevier.