Table 5.
Fuel properties of biodiesel from Y. lipolytica grown on glucose and WCO
| Property/Test | Strain NCIM 3589 on glucose | Strain NCIM 3589 on WCO | Strain NCIM 3472 on WCO | US biodiesel standards ASTM D6751 | European biodieselstandards EN14214 | Indian biodiesel standards IS15607 |
|---|---|---|---|---|---|---|
| Visual test |
+ |
+ |
+ |
NS |
+ |
NS |
| Density (g cm -3)* |
0.81 (0.87) |
1.04(0.87) |
1.19 (0.87) |
NS |
0.8600-0.900 |
0.8600-0.900 |
| Water content (vol %)* |
ND |
ND |
ND |
0.05max |
0.25max |
0.03max |
| TAN (mg NaOH/g)* |
0.2 |
2.8 |
2.3 |
0.8max |
0.5max |
0.5max |
| FFA (%)* |
0.1 |
1.4 |
1.15 |
NS |
NS |
NS |
| Cu strip corrosion* |
Class 1a |
Class 1a |
Class 1a |
Class 3max |
Class 1max |
Class 1max |
| CN*** |
56.6 |
50.8 |
59 |
47-65 |
51 min |
51 min |
| Kinematic viscosity (40°C; mm2/s) *** |
4.44 |
3.6 |
6.44 |
1.9-6.0 |
3.5-5.0 |
3.5-5.0 |
| SN* |
190.81 (194.48) |
256.16 (249.4) |
168.5 (177) |
NS |
NS |
NS |
| IV* |
65.7 (70.64) |
37.8 (47.9) |
54.5 (61) |
NS |
120max |
NS |
| HHV(M J kg-1)*** |
40.39 |
36.77 |
41.25 |
NS |
NS |
NS |
| Concentration of γ-linolenic acid (C18:3) (%)* |
0.1 |
0 |
0 |
NS |
12max |
NS |
| FAME having ≥4 double bonds (%)* | ND | ND | ND | NS | 1 max | NS |
WCO – waste cooking oil. The experimental values are means of three independent determinations.
ND: Not Detected; NS: Not Specified. * Experimentally determined values, followed by predicted ones, if any, in brackets.
** Calculated using experimental SN and IV while the values in brackets determined from predicted ones.
*** Predicted values as mentioned in Materials and Methods.